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Class 10,000 microbial clean room design instructions

1. Microbial clean room design instructions and technical indicators:

(1) Microbiological clean room design description The air conditioning system in the experimental area consists of a combined air conditioning unit with a cooling capacity of **KW.

(2) Microbial clean room design instructions cleanliness (10,000 levels) ≥ 0.5um ≤ 350,000 particles/m3

(3) Microbial clean room design instructions Microbial planktonic bacteria Class 10,000 ≤ 100/m3.

(4) Microbial clean room design instructions: sedimentation bacteria: Class 10,000 ≤ 3/dish.

(5) Microbial clean room design instructions lighting: 300LX for the experimental bench and 150LX for other production areas.

(6) Microbial clean room design instructions: the relative static pressure difference between adjacent rooms indoors is ≥5pa, and the relative outdoor static pressure is ≥10pa.

(7) Microbiological clean room design instructions: indoor temperature is 18-26°C, and indoor relative humidity is 45-60%.

(8) Microbial clean room design instructions indoor noise ≤ 60db (A).

(9) Microbial clean room design instructions Power supply: AC220V/380V-50HZ

2. Microbiological clean room design instructions

The air exhaust outlet is equipped with a high-efficiency filter, and the air supply outlet is equipped with a high-efficiency filter static pressure box to achieve clean air intake. In addition, it is more convenient to replace the high-efficiency filter.

3. Generally, the indoor air supply and exhaust adopts the upward and downward ventilation method. The indoor air exhaust outlet should be located in the area with the highest risk of indoor contamination. It should be arranged on one side without any obstacles.

4. The return air outlet uses a residual pressure valve to automatically adjust the indoor pressure to maintain a positive pressure and clean state.

1. Microbiological clean room design instructions

Design Notes:

The basic ideas of laboratory design are economic and practical. The purification requirement is level 10,000. The laboratory design includes first update, second update, air shower and buffering and other pre-experimental preparations. The principle of separation of people and logistics is adopted to reduce experimental contamination and ensure safety.

A. This layout is compact and reasonable, which meets the requirements of laboratory operation and air cleanliness level. At the same time, it strives to be scientific and economical, taking into account the laboratory requirements and development and optimal operating costs.

B. An air shower is installed at the entrance of the clean room, which can effectively remove dust from the human body and reduce the amount of dust in the clean room. At the same time, the air shower also acts as an airlock to prevent unclean air from entering the clean area through the door. C. The clean room needs to supply a certain proportion of fresh air to the room. After purification and filtration, it enters each laboratory to compensate for the exhaust and ensure positive pressure and staff needs.

D. The number of air return air volume uses the air volume regulating valve to control areas with different requirements for cleanliness, and adjusts the pressure gradient to ensure that the airflow flows from the "clean" area to the "polluted" area, and the pressure difference between different levels of the clean room is ≥5pa , the pressure difference between the clean room and the outside is ≥10pa.

E. The clean room is equipped with a purple light sterilization device.

F. The transfer window is made of stainless steel, mechanical interlocking control, and has a sterilization device inside.

G. The observation window is a closed clean window and the door is an airtight clean door.

2. Problems in the design and construction of biological clean rooms

The application of biological clean rooms is becoming more and more widespread, currently concentrated in hospital operating rooms and wards, pharmaceutical factories, biosafety laboratories, animal rooms, etc. Clean rooms of the same level have very different requirements due to different application fields. Various problems have arisen in several biological clean rooms tested, many of which are caused by a lack of understanding of the characteristics and requirements of various biological clean rooms. .

First of all, different types of biological clean rooms must comply with corresponding specifications. If you do not understand these specifications, you cannot design and construct them. Some current specifications for different types of biological cleanrooms are listed in the table below. Only by being familiar with the characteristics of each industry and fully grasping the requirements of each specification can we effectively carry out the design, construction, and commissioning of biological clean rooms.